Unknown Bacteria Report

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The correct identification of microorganisms is vital in any health care field. The objective of this report is to demonstrate an understanding of the material presented throughout the fall semester of Delta’s Microbiology class. Using applied knowledge and lab techniques learned in BIO203W, students were able to identify an unknown bacterium.

MATERIALS USED
 Unknown bacteria
 Lab coat
 Goggles
 Nitrile gloves
 Clean glass slide
 Gram stain kit Crystal violet, Iodine, Decolorizer and Safranin
 Water for rinsing between stain techniques
 Bibulous paper
 Microscope
 Immersion oil
 Lens paper and lens cleaner
 Inoculating loop
 Bunsen burner
 Igniter
 Pipette and mechanical pipetter  Inoculating needle
 Forceps
 Spot plate …show more content…
2. Motility agar plate (MT) Test
After sterilizing the inoculating needle and dipping it into unknown bacteria #4, the MT agar plate was poked in the center of the media.

3. Fluid Thioglycolate Tube (FT) Test
Aseptic inoculation of the FT tube just below the top surface of the agar was performed using one loopful of unknown bacteria #4.

4. Oxidase Test
Using a TSA media plate and a sterilized inoculation loop, one loopful of unknown bacteria #4 was streaked onto the media plate. The quadrant method was used as the streaking technique.

5. Nitrate Broth Tube (N) Test
Aseptic inoculation of the nitrate broth tube was performed with one loopful of unknown bacteria #4.

6. Catalase Test
Using a TSA media plate and a sterilized inoculation loop, one loopful of unknown bacteria #4 was streaked onto the media plate. The quadrant method was used as the streaking technique.

7. Blood Agar Plate (TSA-B) Test
Using a blood agar media plate and a sterilized inoculation loop, one loopful of unknown bacteria #4 was streaked onto the media plate. The quadrant method was used as the streaking

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