Background Information
Part 1 In the first part of the enzyme lab, we mixed a substrate and an indicator with an enzyme. There was also a neutral buffer in each of the chemical mixtures. The neutral buffer regulated the pH to around 7. We got a color palette and once we mixed each together, we observed and saw a change in the color of the substance. The darker and more brown the substance got, the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased about 10% a minute until it sort of equilibrated at 4 minutes and didn’t change to the fifth minute mark. If we were to change anything we did in the experiment, we would make our comparisons to the chart more precise. Overall we thought it…show more content… 2. We inverted twice to mix the SPBN test tube.
3. We measured 10 mL of neutral buffer (pH 7) with a syringe and disposed it into test tube ENB.
4. We heated up 2.5 mL of turnip peroxidase (the enzyme) to 100° Fahrenheit and disposed it into test tube ENB.
5. We inverted twice to mix the ENB test tube.
6. We disposed the contents of test tube SPNB into ENB using a disposable transfer pipet and inverted twice to mix the solution.
7. In 1 minute intervals, we observed the color changes of the reactions and compared them to the color palette. We did this for 5 minutes.
Data: Tables and Graphs
Time - Minutes pH - Percent
1
60%
2
70%
3
80%
4
90%
5
90%
Rate
0.75
Part 1
Part 1 In conclusion, we wanted to know if oxygen would be produced if we combined a substrate, enzyme, and neutral buffer. We thought if all substrates reacting with an enzyme create a reaction, then there would be oxygen produced because all reactions have a product
