The experiment outlined in this report was carried out with the intention to observe and explore the effects of certain named drugs on a vascular preparation, in the cardiac region specifically. The cardiovascular preparation used in this experiment was an isolation of the descending aorta of a rat. This isolate is particularly useful as it mirrors the responses that would be observed in a human sample, thus this experiment will give rise to conclusions applicable to humans. The drugs which will be tested are as follows: acetylcholine, adrenaline, 5-hydroxytryptamine, isoprenaline, noradrenaline and sodium nitroprusside. As the preparation, inherently was not in a live …show more content…
Table 1. Final bath concentrations used for each drug.
Drug | Final Bath
Concentration | Noradrenaline | 1×10-7 mol/l | Isoprenaline | 1×10-7 mol/l | Adrenaline | 1×10-7 mol/l | Phenylephrine | 1×10-7 mol/l | Acetylcholine | 1×10-6 mol/l | Sodium Nitroprusside | 1×10-8 mol/l | 5-Hydroxytryptamine | 1×10-5 mol/l |
With regard to the second part of the experiment, serial dilutions were produced with a range of concentrations reaching from order 10-9 M to 10-5 M solutions with intermediary steps also included. Experimental procedure mirrored the techniques outlined earlier, rinsing the tissue in between each trial.
Table 2. The results as determined from the graphs drawn by