General Methods Animals. Male Sprague-Dawley rats weighing 200- 350 g at the start of the experiments were used for all experiments and maintained on a 12 hour light/dark cycle with ad libitum access to water. All procedures were approved by the University of Michigan University Committee on Use and Care of Animals (UCUCA). Furosemide Treatment: Once a week for three weeks, rats were given a subcutaneous injection of the diuretic furosemide (20 mg/kg) to induce diuresis and natriuresis, or given a vehicle injection. “VVF” rats were given vehicle injections for weeks 1 and 2 and a furosemide injection for week 3. “FFF” rats were treated with furosemide for all 3 injections. Body weights were recorded pre-injection and 2 hours post-injection to verify excretion. Following treatment rats were food deprived with access to distilled water only for 24 hours. Taste reactivity testing or T-maze testing was conducted approximately 24 hours after furosemide depletion. Following taste reactivity testing or T maze testing, rats were given free access to 3% saline and water in their home cage for 90 minutes. Intakes were recorded and analyzed.…show more content… Rats were either given free access to 3% saline and water (DOCA- WS) or water only (DOCA-NS). The day prior to behavioral testing rats were food deprived with access to distilled water for 24 hours. Following taste reactivity or T-maze testing rats were given free access to 3% saline and water in their home cage for 90 minutes. Intakes were recorded and
