answer ch 7 college chemistry Essay

Submitted By donnabottineau
Words: 604
Pages: 3
Open Document
Lab 2 Report
Gram Staining
Results
40X cell wall
4x10=100
Gram positive

Gram negative this cheek cell is A cluster and its Shape is cocci

100X
10x10=100
Gram positive

Gram negative

400X Nucleus
40x10=400
Gram positive

Gram negative

1. Describe, in detail, the differences in the gram + and gram - bacterial cell membranes. Include number of layers, what each layer is made of, the charge on the membrane etc.

The difference between the gram negative and gram positive are. Gram positive cells contain 3 layers. The first layer are thick layers of peptidoglycan (cell wall), this prevents the cell from lysing. The peptidoglycan contains teichoic acids and lipoteichoic acids. Next you have the gel like material, and the cytoplasmic membrane. With a gram positive staining the cell will dye purple, dye will then dehydrate the cell trapping it and making it hard for it to get out. Gram positive are easier to treat.
Whereas the gram negative are more complex made up of 6 layers or more. They have a thin peptidoglycan that are surrounded by an outer membrane (lipid bilayer), the outer layer of the membrane is lipopolysaccharide they are a live bacteria that protects the cell and stops things from entering such as substances. Next you have the periplasm, the lipoprotein and cytoplasmic membrane (inner membrane lipid bilayer). Gram negative have Lipid A which is an immune defence preventing thing from entering. When staining a gram negative it will turn pink otherwise they would be clear. The Gram negative cells are harder to treat

2. Discuss, at each step of the staining process, how gram + and gram – bacterial behave differently and why.

In each step of the staining process this is how the gram negative and gram positive behaved with each other.
Step one: the gram negatives and gram positives are the same with a little black. Because you are just fixing it.
Step two: colour though out the cells, with gram negative a little lighter.
Step three: The same in this step.
Step four: Gram negative is stripped of the colour where gram positive keeps colour. This is because it is alcohol and the gram positive dehydrates after the crystal violet and making it hard to get out whereas the gram negative is not like that it does not hold the dye you need special dye for this procedure.
Step five: The gram negative dyed pink and the gram positive dyed purple.

3. Discuss the
Show More

Related Documents: answer ch 7 college chemistry Essay

Essay on Confusion: Confucianism and Education

Teaching & Learning Position Paper May 2012 Don Starr 1 ISBN 978‐0‐9570066‐1‐4 China and the Confucian Education Model A position paper commissioned and published by Universitas 21, the leading global network of research universities for the 21st century. May 2012 Don Starr Centre for Contemporary Chinese Studies Durham University Former President of the British Association of Chinese Studies d.f.starr@durham.ac.uk 2 Contents page Executive…

Words 8860 - Pages 36

Ebooks Solution Manuals and Test Banks for Text Books Essay

Dear all, If u need anything from the list, do contact us anytime, we provide competitive prices and fast delivery after payment done. We are assisting students by providing them quality services regarding Solutions and Test Bank for courses. We hope that you people will like our service and get better grades. Please Contact us @ : solvedanswers@gmail.com solvedanswers@gmail.com Use CTRL + F to search the titles you looking for , if the title you searching is not in the list, do not…

Words 19224 - Pages 77