• Prepare a sample from a patient and isolate whole bacterial DNA.
• Make many copies of the desired piece of DNA.
• Sequence the DNA.
• Analyze the sequence and identify the bacteria.
• What kind of patient samples are used for the purpose of identifying possible pathogens?
• What does PCR do, how does it work, and why is it useful?
• How do you separate the desired DNA from all others?
• How does an automatic DNA sequencer work?
• Why is it possible to use a DNA sequence to identify bacteria?
• Automatic DNA sequencer
• Digestion buffer
• Micro concentrator
• PCR machine
• PCR master mix
• Strip tube
Procedure- as stated in lab
1.The S is the Svedberg unit, used to express sedimentation coefficients of biomolecules and cell components. It is essentially an expressed of speed. The smaller the S value, the smaller the molecule moves in a centrifuge.
2. Some bacteria grow better in liquid and some grow better using the molecular method.
3. Supernatant- Floating on the surface, or relates to the clear fluid over a sediment or precipitate. In this lab, used to describe the layer of fluid that is over the centrifuged