Cauliflower Lab Report

Words: 505
Pages: 3
Open Document
In experiment I and III we attempted to take cell fractions of cauliflower, created through a series of three differential centrifugations, and ultimately determine which cell fraction contains the greatest number of mitochondria. The Citric Acid Enzyme succinate dehydrogenase (SDH) is a biochemical marker that allows us to indirectly asses the presence of mitochondria. SDH is an enzyme found in the inner mitochondrial membrane and is responsible for catalyzing the oxidation of succinate into fumarate. The mitochondria can be isolated through differential centrifugation and then treated with a sodium azide regent, a type of cellular poison, to block the normal electron transport system in the cell sample. To measure the activity of the enzyme, an artificial electron acceptor DCIP is used to accept two. Upon receiving electrons, the oxidized DCIP is reduced and the color of the mixture changes from blue to colorless. Spectrophotometry at the 600nm range can then be used to quantify this color change, and give an indication of the mitochondrial …show more content…
Cell fraction E2 (P2) was made from a suspended pellet centrifuged at 2000 x g for five minutes. Cell fraction E3 (P3) was created from a suspend pellet formed from the supernatant of the E2 (P2) cell fraction centrifuged at 21,000 x g for fifteen minutes. Cell fraction E4 (S3) was made form the supernatant of cell fraction E3 (P3). The control, E1 (none) contains no cell fraction. Figure 2 for Project 1: Wet lab 1 – Microscopy & Cell Fractionation, shows that the cell fraction created from the highspeed centrifugation contains mitochondria(intext citation). Therefore, I hypothesize that the cell fraction containing the greatest number of mitochondria will be Cell Fraction E3 (P3). Since the oxidized form of DCIP is blue and the reduced form is colorless the hypothesis can be proven by measuring the change in absorbance at 600nm, and thus the greatest SDH
Show More

Related Documents: Cauliflower Lab Report

Essay on Fuss: Statistical Hypothesis Testing and Experimental Design

2. Style: The Abstract is ONLY text. Use the active voice when possible, but much of it may require passive constructions. Write your Abstract using concise, but complete, sentences, and get to the point quickly. Use past tense. Maximum length should be 200-300 words, usually in a single paragraph. The Abstract SHOULD NOT contain: lengthy background information, references to other literature, elliptical (i.e., ending with ...) or incomplete sentences, abbreviations or terms that may be…

Words 4465 - Pages 18

Essay about 266 Unit 1

266 M1 Obj 1: Define key words RT women’s health care: (pg. 762-763) Adjuvant therapy additional treatments that increase or enhance the action of the primary treatment Adnexa accessory organs of the uterus, such as the fallopian tubes and ovaries Amenorrhea absence of menstruation. Primary amenorrhea is a delay of the first menstruation. Secondary is cessation of menstruation after its initiation Atrophic vaginitis inflammation that occurs when the vagina becomes dry and fragile, usually as a…

Words 7156 - Pages 29

Essay about An Introduction to Hydrophonics and Controlled Environment Agriculture

Introduction to Hydroponics and Controlled Environment Agriculture by Patricia A. Rorabaugh, Ph.D. University of Arizona Controlled Environment Agriculture Center 1951 E. Roger Road Tucson, AZ 85719 Revised December, 2012 TABLE OF CONTENTS CHAPTER 1: Controlled Environment Agriculture and Hydroponics: Past, Present and Future The Plant How to grow greenhouse crops Plant Protection: Insects and Diseases Basic Principals of Hydroponics Transplant Production Pollination, Fertilization and Bee…

Words 40128 - Pages 161