The measuring techniques are as follows: a clean transfer pipette was used to transfer one daphnia, from the daphnia jar to a clean depression slide, which was then transferred onto a microscope. The microscope was focused on the daphnia so that the heart is in view, and then a researcher recorded the number of heart beats for a 10 second interval. This first observation determined the base heart rate of the first daphnia. Daphnia were then removed from the microscope and one drop of the test solution was added to the daphnia. A ten-minute time period was allotted for daphnia HR to adjust to the solution. 3 trails per test solution. Three trails per test solution were done to establish statistical significance; even for the no stimulus solution, i.e. the baseline HR. Daphnia were placed back into aged tap water and allowed to equilibrate for 10 minutes before subjecting the daphnia to a new stimulant. In the Kaas’s work, it suggests that ten minutes was the average amount of time needed for the daphnia to rebound back to normal baseline heart rate. Hence, ten-minute delay between new stimulants was the minimum time allotted to each daphnia before moving onto the next solution.…show more content… Although, the researcher recorded the number of heart beats for a 10 second interval, the BPM was calculated by extending the 10 second interval to 60 seconds, i.e. multiplication by 6. The average HR was achieved by taking the BPM for each trail and dividing by the number of trails, i.e. three. The data was analyzed by plotting the calculated BPM for each test solution, i.e. base HR, trail 1, trail 2, trail 3 and average HR, from each daphnia. This study was designed to sequester the relationship between BPM, i.e. Daphnia magna heart rate and effect of the
