Data Collection and Processing & Conclusion and Evaluation
Data Collection and Processing
When dropping the bead into the different concentrations the beads slowly sank to the bottom, bubbles formed around the bead before it took off.
As the concentration of hydrogen peroxide increased the time that the bead took to rise decreased.
At 1.0molL-1 the bead almost instantly rose to the top once touching the bottom.
Table to Show Averages and Standard Deviation for Time Taken For Bead To Lift Off The Bottom (s) (±0.01) Of The Different Concentrations of Hydrogen Peroxide (±0.1molL-1) During Experiment
Concentration Of Hydrogen Peroxide (±0.1molL-1)
Average Time Taken For Bead To Lift Off The Bottom (s) (±0.01)
Rounded to match uncertainties: 12.70
Calculating Standard Deviation:
Calculated using Excel formula builder
1. Choosing on the formula builder
2. And highlighting the wanted row ie;
3. Excel automatically calculates the standard deviation
Conclusion and Evaluation
From this experiment I can conclude that the concentration of hydrogen peroxide does affect the time taken for a bead to lift off
Both the graph and tables illustrate this point. When looking at the graph one can clearly see the strong relationship between the concentrations of hydrogen peroxide and the time taken for the bead to rise. At 0.2molL-1 on the graph the time taken is the longest and as the concentration increases the time taken for the bead to take. This is again highlighted in the averages table where for 0.2molL-1 the time taken is 12.70 seconds whereas for 1.0molL-1 the alginate bead only took 3.87 seconds. This is a difference of 8.83 seconds highlights that there is a clear difference in the time the alginate bead takes to raise with different concentrations. When looking at my raw data, one will see that my data is fairly accurate with which is clearly shown through the small error bars on the graph and the trend line on the graph touching every point. Furthermore when looking at my table of raw data, it is evident that every time the experiment was repeated my results were fairly similar. 0.6molL-1 and 0.8molL-1 have extremely small standard deviation, unlike, 0.2molL-1 that had the highest standard deviation, as the results ranged from 11.82 to 13.44.
The results of my experiment conclude and support the well known scientific theory. The enzyme catalase is present in the yeast alginate beads, and is used for breaking down hydrogen peroxide into hydrogen and oxygen. When one drops the bead into the hydrogen peroxide catalase diffuses the hydrogen peroxide and creates two products, hydrogen and oxygen as the enzyme reacts with the substrate. The oxygen gathers around the bead and this causes it to rise. Therefore, the higher the concentration the more active sites that are presents for catalase and the substrate to react with causing oxygen to be formed quicker. Thus causing the oxygen to be trapped quicker and gravitate towards the alginate bead causing it to rise. This is confirmed through scientific sources “When the catalase comes in contact with hydrogen peroxide, it turns the hydrogen peroxide (H2O2) into water (H2O) and oxygen gas (O2)” (HowStuffWorks)
Finally, I conducted a standard deviation. For this experiment the standard deviations represented that all of my data within two standard deviations. For this experiment my standard deviations are fairly small indicating that the data collected during my experiment is accurate. Furthermore this is shown through the strong correlation on the graph.
When evaluating my method, I can clearly see that my experiment