Enzymes Under Different Temperature Conditions Lab Report

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Pages: 5
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Objective:
Explain what the enzymes are and their activities under different temperature condition. Introduction:
Macromolecules are polymers that are made up of monomers subunits. Three conventional biopolymers in human body are carbohydrates such as sugar, protein, and nucleic acids such as DNA and RNA.
According to website, Wikipidia.org, Enzymes are macromolecular biological catalysts, which speed up chemical reactions. Where in catalytic cycle, it starts with an empty active site of an Enzyme such as sucrose, and then binds with a specific reactant called substrate. After the binding process the products are made and release, such as glucose and fructose. The enzyme raised the rate of a reaction by cutting the activation energy needed …show more content…
Knowing the how temperature affect the activity of the enzymes will help the metabolic process as well.
Methods:
To get started, took 4 empty test tubes and placed it on a tube rack. Then filled each test tube with 5 ml of 3.0 % Hydrogen peroxide (H2O2) and 3 ml of water (H20); and to make sure not to confuse the test tubes, labeled each test tube by using a wax pencil, with the initials of one member of the group and with test tube labeled number 1, 2, 3, and 4.
First, placed test tube 3 in the water bath, which has been prepared on the side bench in order for it to reach 37 degree Celsius. With test tube 1, in order to make it 0 degrees Celsius,
400 ml beaker was filled with ice and water then placed the test tube in this beaker. Made sure to wait at least 10 minutes for both test tube 1 and 3 to be in their respective temperatures before headed to the next step; and while waited, prepared the Nalgene bottle and the oxygen Gas Sensor. The oxygen Gas Sensor was programed to be at 180 seconds for …show more content…
The test tube 3 was taken off of its 37 degrees Celsius bath and did the same steps taken above. Similar, with the test tube 4, it was taken off of its 55 degrees Celsius bath and did the same steps taken above.
Made sure the entire slope, m, of every reaction was recorded.
The dependent variables were the rate of the oxygen being produced while independent variables were the different temperatures. The controlled variables were the 180 seconds time frame of every reaction, the same size of the Nalgene bottle being used, and the amount of enzyme solution being dropped. Lastly, the basis for comparison was the test tube with 23 degrees Celsius room temperature.

Results:
The graph started with the minimum temperature of the Enzyme at 0 degree Celsius and a slope of 0.012942 oxygen production. Then the temperature of the Enzyme rose to 23 degrees Celsius as the slope rose to 0.016405 oxygen production. The rate of oxygen production reached its maximum at a slope of 0.016873 when the enzyme’s temperature was at 37 degrees Celsius. Finally, the rate of the oxygen production reached its lowest slope of
0.000052027 when the temperature of the Enzyme was at its highest of 55 degrees
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