Nt1310 Unit 9 Lab Report

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Address each of these points in your own words (9 points).

Why is it important to adjust the condenser when you are using a microscope?
It is important to adjust the condenser when using a microscope in order to obtain a clear image of the specimen. The condenser focuses the microscope’s source of illumination and can be moved farther from or towards the stage in order to adjust the intensity of the light source. It is important that just the correct intensity of light be focused to have a clear and detailed image.

Why is it important to adjust the iris diaphragm when you are using a microscope?
The iris diaphragm is usually a disc below the stage that can alter the size of the beam of light that reaches the specimen. This can control the …show more content…
Resolution usually refers to the amount of detail that can be observed in an image. As you move farther away from an object in real life for instance, you lose the ability to see close details and thus lose resolution. On the other hand, magnification refers to the size that an image is being observed at. On a microscope, a 10X lens means that you are seeing an image through the lens at 10X magnification. Resolution is important to magnification because when an image is magnified, it must also have the proper resolution to see the details that are seen …show more content…
You raise the stage all the way to the top under the lowest powered objective before looking through the eyepieces in order to clearly see that the stage has been raised all to the top without hitting the lens and to try and provide a clear initial image. After this coarse adjustment, it is no longer used because the fine focus is then used for minor adjustments.

How does working distance change as you switch from the 4X objective lens to the 10X objective lens?
Working distance is simply defined as the distance between the slide and the lens of the microscope. As you move from a low power objective (4X) to a higher power objective (10X), working distance decreases due to the increased size of the lens and how the lens is closer to the slide.

How does the field of view change as you switch from the 10X objective lens to the 40X objective lens?
The field of view decreases as you switch from a 10X objective lens to a higher power objective (40X) because the image seen is magnified and closer so that you are seeing less of the actual slide (field of view) but the detail is greater.

Why did we stain the cheek cells with a stain (e.g., crystal
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