Overall the experiment was successfully performed, plasmid pBR322 was efficiently isolated from E.coli. The final results obtained after the analysis revealed that differential centrifugation and gel electrophoresis are efficient techniques to purify plasmids DNA and proteins. Based on the equation of the line given by the calibration curve, the calculations showed a value of 4406 bp in plasmid pBR322. When comparing this result with the literature value of 4361 bp it can be seen that the two values are pretty closed to each other, there is just a slightly difference given by a lower experimental value obtained. A relative error of 1.03% was calculated, indicating a pretty good correlation given by this low error. This relative error could be the product of improper plasmid purification in the first part of the experiment and also the inaccurate measurements of the migration distance, first to construct the…show more content… There are different possible reasons or sources of errors for this observed behavior, which are pretty common issues for the Agarose gel. These could be the improper preparation of the gel, if it is not poured correctly it will not solidify or polymerize evenly, which will cause the molecules to smear. Also, overloading the wells could be another possible explanation, if there are filled in excess, or an improper dilution of the sample under study can cause the sample to get out of the well. The improper preparation of the sample can be another source of error. As in any experiment, contamination is one of the major sources of error, if the plasmid pBR322 was not purified successfully and traces of undesired molecules were still present, this could be as well a cause of the present of these weak bands. Lastly, using the wrong buffer, wrong temperature, or wrong conditions could be also counted as possible sources of
