Pglo Transformation Lab Report

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Purpose: This investigation is demonstrating the act of genetic transformation. We inserted a gene into the bacterium E. coli. Gene being a piece of DNA which provides the instructions for making a protein. The main chemical components used in this lab were calcium chloride, E. coli bacterium, a broth (made up of carbohydrates, amino acids, vitamins, nucleotides, and salts that allow bacteria to grow), pGLO plasmid (contains arabinose operon that turns on when the arabinose sugar is present, GFP that glows green in fluorescent light, bla that provides ampicillin resistance, and ori which are plasmid replication genes). A real world scenario of this genetic transformation with certain types of foods and GMO’s. To be more specific when growing corn farmers tend to test corn through genetic transformation to protect it from getting pesticides to help produce faster.

Hypothesis: The plates with pGLO will be resistant to antibiotics making it have growth. The plates with ampicillin and no pGLO will …show more content…
(an antibiotic that kills bacteria). This idea can be proven by comparing the two plates that had both the pGLO and the ampicillin, to the plate that had just the ampicillin. Which in this case is the control of the experiment. In the two plates containing the pGLO, the bacteria was able to grow. In the plate with just ampicillin, none of the bacteria survived. The plates with both pGLO and ampicillin grew differently than the plate with neither ampicillin nor pGLO. Our findings show that arabinose had to be present in order for the bacteria to glow. This is proven by the fact that the only plate that contained arabinose happened to be the only one that glowed under the UV light. In this case the plate with the +pGLO, the LB, and the ampicillin would be the control, for it only had one change between it and the plate that glowed. The difference between the two was

Pglo Transformation Lab Report Essay

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process of transformation was green fluorescent protein. Two tubes were labeled “+pGlo” and “-pGlo” and 250 μl of CaCl was added and tubes were placed on ice. I used a sterile loop and extracted 3 large circular colonies of E. coli then swirled it into the +pGlo tube and repeated this process for –pGlo tube. Under UV light both tubes displayed no color difference. The tubes were placed on ice for 10 minutes then heat shocked at 42 ˚C for exactly 50 seconds in order for transformation to occur with…

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Pglo Transformation Lab Report

Coli Bacteria with the Gene GFP (green fluorescent protein) By Using pGLO As a Vector Introduction: Genetic transformation is the alteration of a cell by taking DNA from another organism and expressing those new characteristics from the organism of which it took the DNA from (Julie Carnagie and Leonard C. Bruno 2006). The cells that are able to take up DNA from other organisms are known as competent. This genetic transformation is used for many different things, from making medicines to DNA cloning…

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Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab, the Green Fluorescent Protein, which is typically found in the bioluminescent jellyfish Aequorea…

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pGLO Transformation Lab In this lab you will perform a procedure known as genetic transformation. This term means change caused by genes; it involves inserting a gene into an organism in order to change one or more characteristics in that organism. We will use E. coli K-12 as our host organism; ie the organism that will receive the gene. The gene we will transfer to E. coli K-12 codes for green fluorescent protein (GFP). The source of this gene is the bioluminescent jellyfish Aequorea Victoria…

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