Statement of the Problem:
What question(s) are you trying to answer?
Include any preliminary observations or background information about the subject
If we use paper chromatography to separate the pigments in spinach leaves, then we will see several different pigments because plants use other pigments besides chlorophyll a (the green pigment) to capture visible light for photosynthesis.
1. What factors are involved in the separation of the pigments?
a. Some factors that may be involved in the separation of the pigments are size of the molecules, their attraction to the paper, and how attracted they are to the solvent.
2. What type of pigment is mainly used for collecting light energy in the plants, and what makes you think this is the main pigment?
a. I think chlorophyll a (the green one) is the main pigment used for photosynthesis because it is the main pigment that gives the plant its color. It was also the widest band of pigment found, so this indicates that it is the most abundant, and must be important in photosynthesis.
What are the roles of the other pigments found in the spinach leaves?
Chlorophyll is a plant pigment, which gives plants a green color and allows them to absorb light for photosynthesis. Chlorophyll mainly absorbs red and blue and violet light which reflect green light.La leaf chlorophyll abundance and their occasional presence in other plant tissues queesas causes plant parts appear green but leaf chlorophyll insome is masked by other pigments. The recognition and extraction of these pigments is yconocimiento interesting to study their properties.  The plant pigments, found in loscloroplastos are otransmiten chemical molecules that reflect visible light, or do both at the same time.The Color of a pigment depends on the absorciónselectiva of certain wavelengths of light and other lareflexión. Constitute sustratofisicoquímico fotosintético.Hay process where different kinds of pigments is based: Top: • Chlorophyll (a, b, c, d and bacteriochlorophylls) verde.Accesorios discoloration. • Carotenoids (carotenes and xanthophylls) of coloraciónamarilla • phycobilins of red and blue and red coloration present in blue-green lasalgas, comprising the edge losCianofitos
Write a possible solution for the problem.
Make sure this possible solution is a complete sentence.
Make sure the statement is testable, an if-then statement is recommended to illustrate what criteria will support your hypothesis (and what data would not support the hypothesis).
Chromatography paper strips
Write a paragraph (complete sentences) which explains what you did in the lab as a short summary.
Add details (step-by-step) of your procedure in such a way that anyone else could repeat the experiment. ●
Measure 2cm vertically from bottom of each strip and draw a horizontal line across paper stripusing a pencil
Using a quarter extract pigments from leaf cells
Place chromatography paper in graduated cylinder
Place chromatography chamber in fume hood and allow chromatograms to develop(Part B)
Turn spectrophotometer to warm up the instrument and set wavelength to 605 nm
Setup an incubation area
Keep two beakers with chloroplasts on ice
Label cuvettes 1-5 respectively
Add 1ml phosphate buffer to each cuvette
Bring spectrophotometer to zero
Obtain unboiled chloroplast suspension and transfer three drops to cuvette 2. Insert inspectrophotometer sample holder.
Obtain unboiled chloroplast suspension and transfer three drops to cuvette 3. Insert inspectrophotometer sample holder.
Obtain boiled chloroplast suspension and transfer three drops to cuvette 4. Insert inspectrophotometer sample holder.
Cover and mix contents of cuvette 5. Insert in spectrophotometer sample holder.
This section should include any data tables, observations, or additional notes you make during the lab.