Potato Catalase Lab

Words: 1274
Pages: 6
Open Document
Purpose
Proteins called enzymes are used to increase the speed of a chemical reaction by reducing the activation energy of a substrate5. In this lab, hydrogen peroxide is added to six different samples of cells; raw hamburger, spinach leaf, raw liver, boiled liver, chalk and potato. The purpose of the lab is to observe the reaction of Hydrogen Peroxide with catalase in animal and plant cells, to compare the activity of catalase in animal cells and plant cells and to analyses the effects of boiling enzymes. Both animal and plant cells are known to contain catalase1-3,6. The lab will test to see which cells, plant or animal, have more catalase.
Abstract
Catalase is an enzyme that is found both animal and plant cells1-3. The …show more content…
Part A
1) Wrap tape around the upper part of the 5 test tubes,
2) Using pen, label five test tubes: hamburger, spinach, raw liver, potato, chalk
3) Measure 14ml of the hydrogen peroxide into the graduated cylinder CAUTION: hydrogen peroxide is highly corrosive, avoid contact with skin, eyes. Mouth, immediately wash contact areas with water.
4) Use the scalpel to slice tiny amount of hamburger, just enough to cover the tip of scalpel, place hamburger on slice on plate
5) Repeat the previous step for spinach, raw liver, potato and chalk. Keep all samples separate on the plate (clean scalpel between uses)
6) Using forceps, gently pick up each sample and place in the appropriately labeled test tube
7) Measure temperature of each test tube using thermometer, record results (remember to clean thermometer between use)
8) Add 2 ml of hydrogen peroxide to each test tube. Record observations (use thermometer to record any changes in temperate)
Part B
1) Wrap tape around 2 remaining test tube, label boiled liver, raw liver
2) Slice small samples of raw and boiled liver is same manner as described in step 4 Part …show more content…
The spinach and chalk had no immediate reaction. The potato’s reaction was intermediate. A catalase reaction with hydrogen peroxide involves the release of oxygen water and heat. This suggest that animal cells have a greater amount of catalase compared to plant cells since the animal cell samples had faster, more intense reactions that released the most heat. Therefore, my hypothesis, If hydrogen peroxide is mixed in a solution with plant and animal cells, then a faster and more intense reaction that releases more heat will be seen in animal cell samples (eg. Raw liver) than in the plant cell samples (eg. Potato), is
Show More

Related Documents: Potato Catalase Lab

Lucas Goldoni Essay

Lucas Goldoni Bio Honors Lab Report The Effect of Enzyme Concentration, pH, and Temperature on the Rate of Reactions Abstract: In the lab we submerged pieces of potatoes within different concentration of Hydrogen Peroxide solution, then changed the temperature of the enviorment, and finally changed the pH of the enviorment. The potatoes acted as the enzymes and the Hydrogen Peroxide solution acted as the substrate in our experiment. We discovered that the effects on enzymes changing these variables…

Words 754 - Pages 4

Investigation Write Up Essay example

THE TIME TAKEN, IN SECONDS, TO PRODUCE 50 CM3 OF OXYGEN WHEN ADDED TO 5G OF CATALASE? Benjamin Atkins Background information. Our body produces Hydrogen Peroxide (H2O2) as a by-product of respiration. It is produced in every single one of our living cells, due to its harmful nature Hydrogen Peroxide must be removed as soon as it is produced in the cell. Therefore, cells produce the enzyme catalase to remove the harmful substance. 1 I hypothesise that the higher the concentration…

Words 1369 - Pages 6

Biology Enzyme Lab Essay

Enzyme Lab Introduction/ Abstract An enzyme is a substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction. They are mainly made up of proteins and can tremendously speed up reactions. E. coli ( a bacterium) has about 1,000 different types of enzymes floating around in its cytoplasm at any given time. Enzymes can be used to join and even break up molecules as shown in the diagram below. (1)…

Words 1495 - Pages 6

Essay on Enzymes: Ph and Hydrogen Peroxide

increasing the chance of the particles colliding. * Source of catalase – depending on the source of catalase you are using can also have an effect on the rate of reaction. There are many different sources of catalase such as potato, liver and spinach. Depending on which source you are using for your catalase can produce different results. I have chosen to investigate the effect of changing the concentration of enzyme on the rate of catalase activity. I have chosen this independent variable because it…

Words 1748 - Pages 7

Potato Enzyme Lab

Abstract An enzyme is capable of increasing rate (speed) of a reaction by lowering the activation energy of said reaction, specifically in our case the enzyme catalase. Like most things in the world, enzymes are subject to their environments and have ideal conditions in which they perform the best. Our experiment tested the performance of catalase under a variety of conditions including fluctuating pH, presence of inhibitors, substrate and enzyme concentrations, and changes in temperature, all of which…

Words 1890 - Pages 8

catalase experiement Essay

Potato catalase: a study of enzyme action Introduction Thousands of chemical reactions take place within living cells of organisms. Many of these reactions can also be carried out in the laboratory, but outside the cell, they are often much slower. To speed up these reactions so they would take place as fast as they do in cells would require temperatures or pHs that are inconsistent with life. How then is it possible for reactions to occur in cells at rates fast enough to meet the needs of…

Words 3950 - Pages 16

bio shizz Essay

magnetic resonance spectroscopy” activities are singled out because they make it possible to see and study their movements that otherwise would be concealed (ScienceDaily, 6 Oct. 2013). Biologists have succeeded in reproducing chemical reactions in the lab to slow them down, and that helps them manufacture inhibitor drugs for different diseases such as high blood pressure. In our body, enzymes come together in greater numbers on the cells where they catalyze a reaction; therefore, examining a blood serum…

Words 1654 - Pages 7

Develop An Understanding Of The Metric System

Lab Report 1 Purpose To develop an understanding of the metric system and use it to measure common objects. To also learn about how to report uncertainty in your measurements. Lab Observations Most flat objects do not consist of uncertainty. When we measured round objects, because of its shape, it contained uncertainty. Lab Answers 1. Using a metric ruler, determine the length of the items in Table 1.1 below: In the final column, you are to estimate your measurement precision. To do…

Words 2299 - Pages 10

Essay about An Introduction to Hydrophonics and Controlled Environment Agriculture

Introduction to Hydroponics and Controlled Environment Agriculture by Patricia A. Rorabaugh, Ph.D. University of Arizona Controlled Environment Agriculture Center 1951 E. Roger Road Tucson, AZ 85719 Revised December, 2012 TABLE OF CONTENTS CHAPTER 1: Controlled Environment Agriculture and Hydroponics: Past, Present and Future The Plant How to grow greenhouse crops Plant Protection: Insects and Diseases Basic Principals of Hydroponics Transplant Production Pollination, Fertilization and Bee…

Words 40128 - Pages 161