Prophase Lab Report

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Prophase I:
The objective of prophase I is to prepare chromosomes for exchange and division of genetic information. At the beginning of prophase I, each duplicated chromosome consists of two sister chromatids attached at the centromere.
Stage one, Leptonema: Leptonema is the first stage of prophase of the meiotic process. During Leptonema chromosomes will visibly condense, this will result in haploid recombination and chromosome pairs with sister chromatids held by non-dividing , single centromeres. This is followed by the zygonema stage.
Stage two, Zygonema: Zygonema is the second stage of prophase in meiosis and is where homologous chromosomes find each other become then associated as pairs (bivalents)
Stage three, Pachynema: Stage three
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During this stage the paired chromosomes will begin to repel each other becoming separated into two pairs of chromatids.
Stage five, Diakinesis: This is the final stage of the prophase of meiosis. The nucleus and nuclear envelope will completely disappear and the formation of spindle fibres will take place.The separation of homologous chromosomes is complete and “crossing over” has successfully occurred.The chromosomes will shorten in preparation for the next stage.

Metaphase I: Metaphase I is the third stage of meiosis and works to ensure chromosome alignment is perfect in the centre of the cell. Metaphase is also considered one of the more time occupying stages as chromosome alignment in some cases may take days to reach the required alignment before entering the next stage ‘anaphase’. The centrosomes are located at opposite poles of the cell with the chromosomes being moved towards the centre. Metaphase is responsible for how chromosomes are aligned with the movement of these chromosomes being controlled by the kinetochore microtubules which work to successfully align the chromosomes using pushing and pulling