Fig. 1. This is a picture of the gel after running the DNA samples of two groups through it. The fifth lane is the control and the last lane (labeled MS) is my own sample. There are two bands showing on the gel: The first is the nicked band and the second is the supercoiled band.
Fig.2. This is a picture of the PCR results. The labeled group is my own sample. The first lane is the control group. The second lane is +RT, which is the positive control sample. It contained reverse transcriptase. The third lane is –RT, which is a negative control. It is missing reverse transcriptase. The fourth lane is –RNA, which is a negative control. It is missing the RNA template. There is only one band in the +RT and its size is approximately 1131. The first four lanes labeled on the figure on the right are the results of the original +RT, -RT, -RNA tubes after running them through the gel.
In the first part, we…show more content… We then measured the concentration of the DNA using a nano-drop spectrophotometer. Finally, we tested the purity of the DNA and tested for denatured DNA strands using Electrophoresis, separates samples by size: the smaller the sample, the faster it travels through the gel. Figure 1 is a picture of the gel after running the DNA samples through it. The bands observed were compared to a control group, which contained a pure sample of the desired size of the DNA that we attempted to isolate. Two bands were observed and they looked very similar to the bands in the control group. The first band is the nicked band and the second one it the supercoiled band. The supercoiled band traveled faster than the nicked band because it is tightly packed and therefore has a smaller surface area than the nicked band, which allows to travel faster than the nicked band. In addition, the nicked band is thinner and less bright than the supercoiled band. We did not
