According to Oscar Wilde “Discontent is the first step in the progress of a man or a nation.” This is expressed in the portrayal of the protagonist Jane Eyre. Jane Eyre is a classic love story written by Charlotte Bronte. The novel is based around Jane Eyre, and her journey through life, from a burdensome orphan child living with her loathsome aunt, to a grown sophisticated lady. Although Jane has matured as a character and tries to create a better life for herself, she still endures misfortunes. The story is based around Jane’s experience at Thornfield as a governess. Thornfield is a large house, with a mysterious and grim atmosphere. Many unexplained and unpleasant events occur during her stay, such as ghostly laughs and dangerous fires. Gothic elements incorporated into the story allow the reader to experience and feel Jane’s hardships. Charlotte Bronte uses setting, imagery and symbols, to express the discontentment in Jane’s life. The setting of the passage produces an eerie and mysterious environment for Jane creating feelings of gloom. It takes place during the dead of night in Jane’s quarters at Thornfield. The name, Thornfield, represents the characteristics of the residence. A thorn is something sharp or stiff, or figuratively, a source of discomfort, and a field describes an open space. Thus the name Thornfield can portray a large dwelling that brings feelings of distress and ache. Thornfield is a gothic element to the story, as it is an old desolate mansion. Few individuals reside at Thornfield making it appear abandoned. Thornfield being a new home to Jane, she is still not fully familiar of her accommodations. “I tried again to sleep; but my heart beat anxiously: my inward tranquility was broken.” (Bronte, chapter XV) Jane is unable to feel at peace in her new home. This is shown by her lost of tranquility. There are mysterious staircases and chambers that she is unaware of “I started wide awake on hearing a vague murmur, peculiar and lugubrious, which sounded, I thought just above me.” (Bronte, chapter XV) The gothic component of Thornfield creates peculiar and unpleasant feelings. The story, taking place during the night, paints an image of complete darkness and uncertainty of Jane’s surroundings. There are dark secrets that need to be brought into light, such as the shady realization that a mysterious woman resides right above her. This setting also reflects the despair Jane encountered as a child. During her time at Gateshead, she faced similar emotions when she was sent to the red room for punishment, “ my heart beat thick, my head grew hot; a sound filled my ears, which I deemed the rushing of wings; something seemed near me; I was oppressed,
the terminal, create a file containing the target sequence using the command "vi target.ali". Paste the target sequence and convert FASTA format into PIR format. Note that an asterisk (*) should be included at the end of the target sequence. Save the file. After that, create a Python script to generate the alignment of the target sequence and two templates. In the terminal, type "vi alignment.py" to insert the script. Run the script using the command “mod9.14 alignment.py”. A file named "target-alignment…
example, that the level of 14
C in the biosphere has remained constant over time. In fact the level of 14
C in the biosphere has varied significantly, and as a result the values provided by the equation above have to be corrected by using data from other sources, using a calibration curve, which is described in more detail below. For over a decade after Libby's initial work, the accepted value of the half-life for 14
C was 5,568 years; this was improved in the early 1960s to 5,730 years, which…
software applications use the name "DOC" in combination with other words (such as the name of software manufacturer) for different file formats. As an example, on the Palm OS, DOC is shorthand for PalmDoc, a completely unrelated format (commonly using PDB filename extension) used to encode text files such asebooks.
De facto standard
1. Jump up^ "Registion of a new MIME Content-Type/Subtype - application/msword". IANA. 1993-07-22. Retrieved 2012-06-20…
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Organism specific databases
There are three main nucleotide databases that form the International Nucleotide Sequence Database Collaboration: GenBank, EMBL, & DDBJ. These databases exchange information routinely.
Protein databases include Swiss-Prot, PDB, & sequences that were created from translations of coding regions in DNA sequences stored in GenBank.
GenBank serves as a public access repository for all available DNA sequences. Submitting scientists retain complete editorial control over…
-Guy proved that can denature it and protein can refold (I majorly summarized)
c. Scale of hydropathy – large the number, the more likely it is to be found on the interior of the protein (table…)
d. For given protein you can do a plot using the primary AA sequence of those hydropathic (hydrophobic) numbers and
e. Fig. 6.27 More about primary sequence providing driving force
C. Side chains
a. Interior of protein
b. Absence of water
c. Hydrophobic interactions