+ Pglo Lab Report

Words: 1254
Pages: 6
Open Document
51
Before collecting data and analyzing your results answer the following questions.

1. On which of the plates would you expect to find bacteria most like the original non-transformed E. coli colonies you initially observed? Explain your predictions.

Plates 3 and 4 should contain bacteria that are most like the original non-transformed E. coli colonies. This is because these plates serve as control plates. They do not contain the pGLO plasmid that causes genetic mutations or transformations. Plate 3 should not contain any bacteria because it has ampicillin, which prevents bacteria growth, but plate 4 should contain bacteria because it does not contain ampicillin.

2. If there are any genetically transformed bacterial cells, on which plate(s) …show more content…
Carefully observe and draw what you see on each of the four plates. Put your drawings in the data table in the column on the right. Record your data to allow you to compare observations of the “+ pGLO” cells with your observations for the non-transformed
E. coli. Write down the following observations for each plate.

See image below question 4.

2. How much bacterial growth do you see on each plate, relatively speaking?

Plates 1 and 2, which contained the pGLO plasmid, contained a large amount of bacterial growth. Plates 3 and 4, which served as the control plates and did not contain the pGLO plasmid, differed in that Plate 3 did not contain much evidence of bacteria growth and plate 4 showed signs of some bacterial growth. This makes sense because plate 3 contained ampicillin, which prevents bacterial growth, while plate 4 did not. Relatively speaking, plates 1 and 2 had a lot of bacterial growth, plate 3 had little to no bacterial growth, and plate 4 had noticeable bacterial growth.

3. What color are the bacteria?

Plates 1, 2, and 3 showed signs of bacterial growth. Plates 1 and 3 had clear bacteria. Plate 2 had green, fluorescent bacteria. This makes sense because plate 2 contained arabinose, allowing the bacteria to produce Green Fluorescent Protein …show more content…
Do you observe some E. coli growing on the LB plate that does not contain ampicillin or arabinose? Yes. Plate 4 shows bacterial growth and does not contain ampicillin or arabinose.

1. From your results, can you tell if these bacteria are ampicillin resistant by looking at them on the LB plate? Explain your answer.
The results show that plates 1 and 2 are ampicillin resistant because bacteria continued to grow with the +pGLO plasmid inside the plate. This indicates that the bacteria in plates 1 and 2 are genetically transformed, because they continued to grow even in the presence of ampicillin. It is important to note that the experiment cannot fully prove that the bacteria are ampicillin resistant. These bacteria were in a controlled environment, including controlled temperature and sealed plates. The bacteria would need to be tested in a multitude of environments several times before they could be identified as ampicillin resistant.

2. How would you change the bacteria’s environment—the plate they are growing on—to best tell if they are ampicillin
Show More

Related Documents: + Pglo Lab Report

Pglo Lab Report

centrifuge tubes were individually labeled –pGLO and +pGLO and then placed into a foam tube rack. Each tube was then opened and given approximately 250 µL of the transformation solution (calcium chloride), through the use of a micropipetter (with a fresh tip for each). Subsequently both tubes were closed and placed directly in a tub of ice. A single colony of bacteria was then picked up from the starter plate by a sterile loop and placed in the +pGLO tube. The loop was twirled in the transformation…

Words 496 - Pages 2

Pglo Lab Report

Purpose The overall purpose of the pGLO lab was to transform E. Coli bacteria by adding materials such as luria broth, ampicillin, plasmid, and transformation solution. Transformation is a process in which one strain of bacteria is changed by a gene or multiple genes from another strain of bacteria. Procedures The first thing done for this lab was prepping the plates. Students labelled the plates, and then teachers put in agar agar in each plate, adding either pGLO plasmid, ampicillin, or arabinose…

Words 980 - Pages 4

Pglo Lab Report

holder/float, container of crushed ice, marking pen, copy of guide, microcentrifuge tubes, rehydrated pGLO plasmid, 42*C water bath and thermometer, UV light, 37*C incubator, 2-20 ul adjustable volume micropipets, and 2-20 ul micropipet tips. First, you need to label 2 micro test tubes +pGLO and -pGLO. This needs to also have your groups initials or names if you are doing a class experiment. If you are doing a lab experiment for work, you should put…

Words 1001 - Pages 5

Pglo Transformation Lab Report Essay

Transformation Lab Report Introduction Transformation is the transfers of virulence from one cell to another, through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith, a British microbiologist. Griffith observed that the mutant form, non-virulent form, of the bacteria Streptococcus Pnumoniae could be transformed into the normal, virulent form, when injected into mice along with heat killed normal forms. He concluded that somehow…

Words 1455 - Pages 6

+ Pglo Transformation Lab Report

The protein used in process of transformation was green fluorescent protein. Two tubes were labeled “+pGlo” and “-pGlo” and 250 μl of CaCl was added and tubes were placed on ice. I used a sterile loop and extracted 3 large circular colonies of E. coli then swirled it into the +pGlo tube and repeated this process for –pGlo tube. Under UV light both tubes displayed no color difference. The tubes were placed on ice for 10 minutes then heat shocked at 42 ˚C for exactly 50 seconds in order for transformation…

Words 200 - Pages 1

Pglo Transformation Lab Report

Transforming E. Coli Bacteria with the Gene GFP (green fluorescent protein) By Using pGLO As a Vector Introduction: Genetic transformation is the alteration of a cell by taking DNA from another organism and expressing those new characteristics from the organism of which it took the DNA from (Julie Carnagie and Leonard C. Bruno 2006). The cells that are able to take up DNA from other organisms are known as competent. This genetic transformation is used for many different things, from making medicines…

Words 1092 - Pages 5

Pglo Transformation Lab Report

We inserted a gene into the bacterium E. coli. Gene being a piece of DNA which provides the instructions for making a protein. The main chemical components used in this lab were calcium chloride, E. coli bacterium, a broth (made up of carbohydrates, amino acids, vitamins, nucleotides, and salts that allow bacteria to grow), pGLO plasmid (contains arabinose operon that turns on when the arabinose sugar is present, GFP that glows green in fluorescent light, bla that provides ampicillin resistance,…

Words 1123 - Pages 5

Pglo Transformation Essay

Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab, the Green Fluorescent Protein, which is typically found in the bioluminescent jellyfish Aequorea…

Words 1840 - Pages 8

Lab Report Guidelines Essay

GUIDELINES FOR LAB REPORT Concise and Informative Title that Reflects The Findings of the Experiment [Arial font; 12 pt, bold, centered) A. Author and B. Author (MARB 301, Section 40x) [Arial font; 10 pt, bold, centered] Texas A&M University at Galveston, Genetics Lab (MARB 301), 200 Seawolf Parkway, Classroom Laboratory Building, Galveston, TX 77553 Summary Provide a brief summary of your findings in general terms that will entice me to read on  [Font: Arial, 10 pt*, left aligned] Up to 100 words…

Words 478 - Pages 2

Essay about Pglo Transformation Lab Final 1

pGLO Transformation Lab In this lab you will perform a procedure known as genetic transformation. This term means change caused by genes; it involves inserting a gene into an organism in order to change one or more characteristics in that organism. We will use E. coli K-12 as our host organism; ie the organism that will receive the gene. The gene we will transfer to E. coli K-12 codes for green fluorescent protein (GFP). The source of this gene is the bioluminescent jellyfish Aequorea Victoria…

Words 3220 - Pages 13