Agarose Gel Electrophoresis

Words: 679

Pages: 3

Open Document

The purpose of this experiment was to replicate DNA by using Polymerase chain reaction by observing and analyzing it by agarose gel electrophoresis
Genetic transformation is defined as the process where by a plasmid is introduced into a cell such that when the cell reproduces, it also makes a new copy of the plasmid. Plasmid is defined as a circular DNA particle that is within a microorganism and has a replicating quality that are autonomous. Escherichia coli, is a bacterium that is commonly used for genetic transformation in the laboratory because the E. coli bacteria is a single-celled organism that contain multiple plasmids which are commonly used for such process (Marcus 2018). As the bacteria repeatedly increase in population size, the overall number of plasmids also multiply because the plasmid contains its own replication originally. This new DNA that is introduced can also replicate freely based on the original bacteria chromosome. Genes of variable traits that benefit the organism are contained in the Plasmid DNA. For a culture of only transformed plasmids, there is a need for the organism to only keep the transformed plasmid. This transformed plasmid has genes that codes for protein which protects the cell from …show more content…
Large fragments of DNA move slower whiles small fragments will move faster. Since DNA is negatively charged, it tends to move towards the positive direction. “Increased attraction by the magnetic force also allows lighter molecules to travel faster.” (Serwer 1983). Since the gel box is polarized, the molecules migrate from one end to the positive side when electricity is pumped through the box. This technique allows scientist to test, purify and compare the distance traveled by the size and can prove the absence or presence of genetic transformation in DNA fragments in molecular

Agarose Gel Electrophoresis

Agarose gel electrophoresis is the most effective and easy way to determine various sizes and mobility of unknown DNA samples. This method is generally used in biochemistry, genetics, clinical chemistry, cell molecular biology, and forensics. Before a gel is conducted, PCR must be used to produce exponentially large amounts of specified DNA. Forensic analysis of DNA is important in investigations to help exonerate innocent individuals and link biological evidence to the crime scene. Through the…

Words 501 - Pages 3

Dna Lab Essay

learn more about DNA. Materials: Materials for the first procedure include a microwave oven, one agarose pour, one comb, electrophoresis gel tray, one roll labeling tape, micropipette, micropipette tips, aluminum foil, food dye in micro-tubes, plastic bags or wrap, lab write-up, and toothpicks. Materials for the second procedure are three power supplies, one electrophoresis chamber, one prepared gel, one micropipette, micropipette tips, TBE buffer, and a micro-tube rack with DNA tubes. Materials…

Words 500 - Pages 2

Essay on Gel Elctrophoresis

What is gel electrophoresis ? It is simply used to separate macromolecules like DNA, RNA and proteins. Scientist use electrophoresis whenever they need to sort DNA strands by length. Its also used for seperating other types of molecules like proteins. The gel that seperates the DNA strands is made of of a jell-o type sponge with small holes. Using an electric field, DNA can be made to move through a gel.The electric field consists of a negative charge at one end which pushes the molecules through…

Words 707 - Pages 3

Pbr322 Lab Report

Overall the experiment was successfully performed, plasmid pBR322 was efficiently isolated from E.coli. The final results obtained after the analysis revealed that differential centrifugation and gel electrophoresis are efficient techniques to purify plasmids DNA and proteins. Based on the equation of the line given by the calibration curve, the calculations showed a value of 4406 bp in plasmid pBR322. When comparing this result with the literature value of 4361 bp it can be seen that the two values…

Words 539 - Pages 3

- Coding Region In Chromosome 1 (PMCT118, And Electrophoresis

Amplification of a Non-Coding Region in Chromosome 1, “pMCT118,” and Electrophoresis ------------------------------ Dr. Kinal Genetics Lab Abstract During this experiment, DNA from cheek cells was collected from saline rinses performed by each student in class. The cheek cells were collected by centrifugation which was followed by being resuspended in a Chelex solution. Samples of squamous cells were lysed by being boiled, which resulted in free chromosomal DNA. From here, the samples were…

Words 836 - Pages 4

Essay on DNA diffusion

analysts to prove that a certain individual has been at the certain area, say crime spot, by obtaining DNA sample that has been taken from the crime scene and comparing it with sample DNA taken from several individuals. The method used here is Electrophoresis. This is based on the principle that DNA is comprised mainly of negative charges and they are restricted in different lengths. So, when electric current is passed through it, they move towards the positive side and the lengths of movement also…

Words 2665 - Pages 11

Essay Dna Profiling Using Gel Elecrtophoresis

process of the DNA profiling using gel electrophoresis is something tedious, but quite relevant. The materials that are used in this experiment are, first, the gel mold, the electrophoresis, a micro pippeter and micro pippet tips; a pippeter, a waste beaker, and a disposable pippet. The solutions that are used for this experiment are TAE Buffer solution, and of this is needed about 7.5 mL (50 x concentrate), 382.5 mL of distilled water, and 3 grams of Agarose powder. The procedures are the following:…

Words 937 - Pages 4

Electrophoretic Assay Lab Report

statistical analysis (Avise, Nelson & Prodohl 1996). Application of molecular genetic assays was utilized to identify a sample of tissue with an unknown identity. Initially, deoxyribonucleic acid (DNA) was extracted and assayed in an electrophoretic gel for necessary content (Campbell, Harriss, Elphinstone, & Baverstock 1995).…

Words 2320 - Pages 10

Green Fluorescent Protein Lab Report

(rGFP) from E.coli Strain using Ni+2 - Agarose Affinity Chromatography Abstract Green fluorescent protein(GFP) is a unique bioluminescent protein found in the light organs of Aequorea victoria, commonly known as crystal jellyfish, and which will have broad uses in cell and molecular biology as a reporter of gene expression. The purpose of this experiment was to express a recombinant form of GFP in the E.coli strain BL21(DE3) and purify it using Ni+2 agarose affinity chromatography technology. The…

Words 1457 - Pages 6

Eukaryotic Lab

Our Aim: Our aim is to study the effects of adding a mutant to eukaryotic cells. The four parts of this experiment include finding the number of yeast cells provided, collecting RNA by using gel electrophoresis, synthesize cDNA, and determine the mutant by running a PCR. Backgroung information: The budding yeast is an ideal experimental organism for genetic research. The yeast shares a common life cycle and cellular architecture with higher eukaryotes, and as a microorganism it is easily propagated…

Words 1315 - Pages 6