Blood Typing Lab

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Blood typing is a serious matter, because you hold the patient’s life in the palm of your hand and if he/she is given the wrong type of blood it can result in blood clotting throughout the entire body, which can be fatal. This lab helps demonstrate not only the importance but also how you can realistically determine a patient’s blood type through testing. Performing these objectives helped me to understand blood typing by determining the ABO and RH blood type of four patients. Throughout this experiment, we will learn about actual blood typing procedure, observing how antigen/antibody reacts to the simulated blood, and recognize the requirements for blood transfusion. First and foremost, students need to comprehend the different blood types. …show more content…
Blood typing detects the presence or absence of these antigens to determine a person's ABO blood group and Rh type. For example, people whose red blood cells have A antigens are in blood group A, those with B antigens are group B, those with both A and B antigens are in group AB, and those who do not have either of these markers are in blood group O. If the Rh protein is present on the red blood cells, a person's blood type is Rh+ (positive); if it is absent, the person's blood is type Rh- (negative). Our bodies naturally produce antibodies against the A and B antigens that we do not have on our red blood cells. For example, a person who is blood type A will have anti-B antibodies directed against the B antigens on red blood cells and someone who is type B will have anti-A antibodies directed against the A antigens. People with type AB blood have neither of these antibodies, while those with type O blood have both. So the question states, “How can we determine a person’s blood type through blood-clotting tests?” Well, we need to first recognize what agglutination and non-agglutination look …show more content…
Then, we put the samples on the stage of a stereoscope and noted the basic appearance of the blood was normal and had no change. Second, we were to perform the same procedure as done previously; however, this time we were to perform positive control tests. This resulted in the blood agglutinating of the blood. Third, we tested the blood of patient 1 for A, B, and Rh antigens and we were to observe and record whether or not there was agglutination or no reaction. As seen in my data table I found that patient 1 blood type B-. Next, we examined patient’s 2 blood and according to my data patient 2 has type AB- blood. Then, we tested patient’s 3 blood and figured out that he/she has A+ blood. Finally, patient 4 has tested for O+ blood. The data from the experiments demonstrate that since there is no O– blood Patient 1 can only receive the B– pint, since there is no O– blood Patient 4 can only receive the O+ pint, and the only remaining blood is B– and AB–. Therefore, Patient 2 can only receive the AB– pint and Patient 3 can only receive the A+
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