A compound light microscope would be used to observe very small objects or organisms on a slide. This microscope magnifies up to 1,000 times and is used for general use such as; shape, size, number, arrangement, and overall appearance. To study the internal structure of a minute specimen you would need to use a transmission electron microscope (TEM). This microscope magnifies up to 100,000 and can see internal details of cells.
2. List two jobs where microscopes are used. Describe in what capacity they are used. (2 points)
1. Forensic science technician- They work in crime laboratories and use microscopes to analyze evidence like hair and fibers of clothing.
2. Microbiologists- They use microscopes to study organisms that are too small for the naked eye to see such as bacteria.
3. Why is a special paper used to clean the lenses of a microscope? (1 point)
If the lens needs to be cleaned, only lens paper should be used because paper towels or cloth may scratch the lens.
4. State the name of each of the tagged parts on the microscope shown above in Step 1 of the Procedures: (11 points)
a. Ocular lens
b. Body tube
d. Objective lenses
h. Coarse focusing knob
k. Fine focusing knob
5. What type of microscope has primarily been used in this lab? (1 point)
A compound light microscope.
6. State the function of the following parts of the microscope: (3 points)
a. Iris diaphragm- Controls the amount of light entering the condenser.
b. Ocular lens- Remagnifies the image formed by the objective lens.
c. Condenser- Focuses light through the specimen.
7. State how to determine the following functions of the microscope (2 points)
a. Diopter- used to help focus the microscope and adjust both the left and right eye, found at the base of the left ocular.
b. Interpupillary distance- The distance from the center of one pupil, to the center of the other pupil.
8. What is the total magnification when using a compound microscope with an objective lens of 10X and an ocular lens of 4X? Show the calculation used to derive the answer. (2 points)
Objective 10X times Ocular 4X = Total Magnification 40X
9. Why are coverslips used to observe specimens under the microscope? (1 point)
Coverslips are used to draw out excess water to create a wet mount. Wet mounting makes the specimen flatter which makes it easier to observe.
10. Letter "e" exercise:
a. Describe what happens to a letter "e" when placed under the microscope. How does this compare to its appearance in the newspaper? (2 points)
When placed under the microscope the letter e is upside down and only the side is visible. Compared to the newspaper appearance, the microscope image is clearer and more coarse and grainy.
b. If the slide is moved to the right with the mechanical stage, what direction does the specimen move while observing it under the microscope? (1 point)
If the slide is