Daphnia Lab

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The Environment, Daphnia, and The Intruder.

Abstract. The purpose of the experiment was to prove how pollution and runoff affected our water, food chains, as well as our ecosystem. The subjects used were daphnia magna, obtained from Carolina Research. The daphnia were placed in separate tanks and were cultured for one month, after the month and having approximately 3500 in each tank, 40 daphnia were extracted from each tank and placed in separate cups, and the experiment began. In the more basic cup, (Tank 3, “Green Jar”), at a PH of 7.8, and in the span of 30 minutes, 87.5% of the daphnia died. And in the more acidic jar (Tank 1, “Blue Jar”), at a PH of 6.8, and in the span of 30 minutes, only 45% of the culture did not survive.

I …show more content…
My hypothesis is that the effects of waste drifting in the ocean can affect the aquatic ecosystem, by changing the toxicity/PH, which then affects the land life and the top predators, and their ecosystems

II . Materials and Methods
Exactly forty daphnia in three different tanks kept in the exact same conditions were extracted and put into three different containers. One was a control jar, or the “red jar”, which was left alone. The other two were labeled the “blue jar” and the “green jar.” In the “green jar” the PH/toxicity was raised, to 7.8, with one teaspoon of vinegar. And a timer set for thirty minutes, and checked every five minutes was started.
In the “blue jar” the PH/toxicity was lowered to a 6.8, with one teaspoon of baking soda. And then the timer for thirty minutes was started, checking the clock every five minutes.

I hypothesized that the effects of waste drifting in the ocean can affect the aquatic ecosystem, by changing the toxicity/PH, which then affects the land life and the top predators, and their …show more content…
III. Results & Discussion
(See Figure One for all of these data points.)
In the “blue jar” towards the beginning, four died. At the ten minute mark there was no change in death number but they migrated to the back left corner and then they dispersed at twelve minutes. At the fifteen-minute mark they are still up against the left wall, but they are not spread out. An observation made was that by adding baking soda makes the food float and created a lot of commotion in the middle/left side of the tank. There was no change at the twenty-minute mark.

The “green jar” had contents that within the first five minutes, ½ of the daphnia were already dead. At the ten-minute mark there were none dead, but the daphnia swarmed the front right corner towards the bottom. At the twenty minute mark there was no change. At the twenty-five minute mark eight more daphnia died. Although an observation was that when you add vinegar or raise the toxicity, destroys the food and makes the daphnia very active/crazy with even more erratic, and quicker
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