Escherichia Coli Lab Report

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Effects of antibiotics on Escherichia coli

ANSWER ALL TEN QUESTIONS IN THE SPACES PROVIDED

1. Explain why bacteria are susceptible to antibiotics but the human host is not susceptible in bullet point form. (2/25 marks)

Bacteria are susceptible to antibiotics and not human hosts because antibiotics are designed to target features which are unique to bacteria.
Human cells do not contain a cell wall where as bacteria do, through targeting specific receptors present in cell walls; or involved in their production such as ampicillin; bacteria can be specifically targeted.
Another example of this involves targeting organelles which are specific for bacteria: ribosomes differ in structure between eukaryotes (80S) and bacteria (70S); through targeting …show more content…
Present the results of your viable count data as a Table (4/25 marks ).

8. From the results of your viable count experiment and your growth curve, what can you conclude about the effects of antibiotic W and X on the growth of E. coli ? (4/25 marks)

The results from both the growth curve and viable count data produced in this experiment clearly indicate the influence antibiotics W and X have on E.coli growth. When compared to the control values antibiotic X appears to suspend cell division, with no further growth being seen after introduction of the antibiotic two hours into the experiment; this indicates antibiotic X has a bacteriostatic effect on E. coli growth. Antibiotic W appears to induce cell death as the number of visible colonies after 4 hours is significantly reduced from both the control and the level number after two hours when the antibiotic was introduced; therefore can see antibiotic W had bactericidal effect.

9. Considering your answers Q8 and the patterns of results that you generated what are the real identities of antibiotic W and X?_(1/25 mark)

Antibiotic X is Chloramphenicol, this can be seen as the bacteriostatic effect which was seen in response to X within this investigation is consistent with

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