Nt1310 Unit 3 Lab Report

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3.1.5.3. Coverslip culture technique
Coverslip culture technique was used for reviewing the morphological characteristics of the marine actinomycetes isolates (Pridham et al., 1958). Sterile glass cover slips were inserted at an angle of 450 into solidified SCA medium in petri plate. A loopful of inoculum of each isolate was streaked laterally to the line where the coverslip come across the agar and then the plates were inoculated at room temperature (28±200C) for 7 days. After the development of the mycelia on the coverslip, it was detached and placed on a glass slide for complete observation of structure and arrangement of the spore chain on aerial mycelium, spore morphology, spore bearing hyphae, using a phase-contrast microscope and photographed. …show more content…
Gram staining
A thin smear of selected marine actinomycetes were air-dried and heat fixed to a clean glass slide. The slide was then flooded with crystal violet for about a min and rinsed with tap water. Gram’s iodine solution was added and kept for a min. The slide was rinsed with tap water. The smear was decolorized with gram’s decolorizer and immediately washed with tap water, blot dried and identified under a light microscope.
3.1.5.5. Acid fast staining
The actinomycetes colony was studied by acid fast staining procedure. To a clean glass slide, a thin smear of the isolate was made on and heat fixed and then added with carbol fuchsin. It was heated gently till the steam raised and the process was repeated for three to four times in the course of 5 min, decolorized with acid alcohol for 15-20 seconds, then the slide was rinsed with water and the smear was counter stained with methylene blue for 30 seconds. Lastly, the smear was washed with running water and air dried.

3.2. BIOCHEMICAL CHARACTERIZATION (Shirling and Gottlieb,

Nt1310 Unit 3 Lab Report

The pitch is angled triangular, as this is a preferred method Sinnott (2005). (Eq. 3.4 6) is used to calculate the number of holes per tray: The number of holes per tray above feed stage is 25912, while below feed stage the number of holes is 24793. The choice of hole diameter is usually about 5mm, but an increase in the size of the hole can be considered when fouling causes concern (Sinnott, 2005). The minimum hole size is about twice the size of plate thickness for stainless steel, in this case…

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Nt1310 Unit 3 Lab Report

of 18 test tubes, placing 3 test tubes in each of the 6 test tube racks; one test tube rack for each variable. This includes 3 test tubes for each variable to find an average and reduce the effect of anomalies making the results more reliable. 2. Then using a pipette extract 4ml of algal solution (Tolypothrix) from the beaker and place in a test tube. Repeat this for all 18 test tubes making sure that the measurement is read from the meniscus to ensure reliability. 3. After this, using a new pipette…

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Nt1310 Unit 3 Lab Report

Output folder filepath: E:\Gwc9 File names: 1) lab5.mxd 2) lab5.shp 3) lab5.img Process Details Exercise 1 NOTES 1. Open up Arcmap and ERAS imaging and add roster layers from the satellite. Add band six then examine after that the person will remove and six and then add band for which is the near infrared band for arc map. In ERAS imaging a person can use multiple windows and open up the same file in different bands this is useful to identify different objects. Q1: Briefly…

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Nt1310 Unit 3 Lab Report

Basics of a-Si:H/uc-Si:H tandem solar cells Figure 1.2 shows the scheme of the one of the possible configuration for the thin-film tandem solar cell, which consists out of three blocks: the upper thin-film part, the thick layer of Silicon wafer and the bottom thin-film part, which are built in stack. Figure 1.2. The schematic view of the HIT solar cell According to Schockley the energy of photons, which exceeds the band gap is lost due to the thermalization of the charge carriers to the band edges…

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Nt1310 Unit 3 Lab Report

flow rate. The fluid flows through hose pipes (0.5in.) by a pump (0.5 hp and 30-40 m head) from the tank to the radiator at the flow rate range 500-550 LPM. The total volume of the circulating fluid is 3lts and constant in all the experimental steps. 3.…

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Nt1310 Unit 3 Lab Report

This manuscript developed a HIF-1α stabilizing wound dressings by incorporating dimethyloxalylglycine (DMOG) in electrospun core-shell nanofibers. The authors showed the advantages of the material fabricated by the characterization, the release test, cell culture experiment and animal experiments. However, the reviewer thought that the detailed description of electrospinning process is incomplete. Following the precise analysis of the structure and the amounts of drug incorporated in fibers, the…

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Nt1310 Unit 3 Lab Report

Diaphragm h. Coarse focusing knob i. Illuminator j. Base ~~5. What type of microscope has primarily been used in this lab? (1 point) The compound light microscope was used in this lab. ~~6. State the function of the following parts of the microscope: (3 points) A. mechanical stage- This is the area where a specimen is placed on the microscope for viewing. B. ocular lens- This is used to magnify the image that has been formed…

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Nt1310 Unit 3 Lab Report

10. When will you be required to use the spill kit in a healthcare setting?  The spill kit is used for cleaning the spills like bloods, body fluids, chemical spills or other unknown liquid spills. 11. You walk into the patient’s room and find that the person’s Catheter bag is leaking and there is urine on the floor beside the patient’s bed. Describe how you would respond and what is the process for cleaning up the spill?  The procedure for cleaning up a leaking catheter bag is: • Must wear appropriate…

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Nt1310 Unit 3 Measurements Lab Report

Purpose: The purpose of this lab is to be able to properly use all units of the metric system, and be able to convert the metric units into those that are similar. In order to identify which metric system is being used, it is determined by the measuring device in which is supplied: Thermometers read degrees celsius, graduated cylinders read volume; milliliters, and two forms of weight devices similar to scales called Analytical Balance and Triple Beam Balance in which measure mass. When reading,…

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