Nt1310 Unit 3 Lab Report

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This manuscript developed a HIF-1α stabilizing wound dressings by incorporating dimethyloxalylglycine (DMOG) in electrospun core-shell nanofibers. The authors showed the advantages of the material fabricated by the characterization, the release test, cell culture experiment and animal experiments. However, the reviewer thought that the detailed description of electrospinning process is incomplete. Following the precise analysis of the structure and the amounts of drug incorporated in fibers, the discussion from biological points should be required.

1. Why was collagen blended to PCL? The authors should explain the reason and the implication for collagen.
2. Describe the detailed condition, including the diameters of core and shell nozzle diameters …show more content…
Describe the observation conditions of SEM, including the coating conditions and the applied voltage.
6. Fig. 1D. How did the authors identify the core-shell structure by SEM?
In the reviewer’s consideration, it is very difficult to detect the significance difference of the contrast of the secondary electron scattering between core material and shell material which are identical except 0.1% DMOG.
7. Fig.1D. The diameter of an observed fiber was approximately 1 micron, although the averaged size was 391 nm. In addition, the background of this image was film-like, which looks the beaded region in the electrospinning. The authors should show the distribution of the fiber diameter and provide the representative picture.
8. How is the completeness of the core-shell structure? Is there any fibers which are composed of core solution solely, shell solution solely, or mixture of core and shell solutions? The authors should show the experimental evidence of the core-shell structures.
9. The reviewer considers that collagen embedded in the fibers might be eluted in the medium because it is denatured in HFIP into gelatin form. The authors should show the morphological stability, including fiber diameters, after immersed in the

Nt1310 Unit 3 Lab Report

The pitch is angled triangular, as this is a preferred method Sinnott (2005). (Eq. 3.4 6) is used to calculate the number of holes per tray: The number of holes per tray above feed stage is 25912, while below feed stage the number of holes is 24793. The choice of hole diameter is usually about 5mm, but an increase in the size of the hole can be considered when fouling causes concern (Sinnott, 2005). The minimum hole size is about twice the size of plate thickness for stainless steel, in this case…

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Nt1310 Unit 3 Lab Report

of 18 test tubes, placing 3 test tubes in each of the 6 test tube racks; one test tube rack for each variable. This includes 3 test tubes for each variable to find an average and reduce the effect of anomalies making the results more reliable. 2. Then using a pipette extract 4ml of algal solution (Tolypothrix) from the beaker and place in a test tube. Repeat this for all 18 test tubes making sure that the measurement is read from the meniscus to ensure reliability. 3. After this, using a new pipette…

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Nt1310 Unit 3 Lab Report

Output folder filepath: E:\Gwc9 File names: 1) lab5.mxd 2) lab5.shp 3) lab5.img Process Details Exercise 1 NOTES 1. Open up Arcmap and ERAS imaging and add roster layers from the satellite. Add band six then examine after that the person will remove and six and then add band for which is the near infrared band for arc map. In ERAS imaging a person can use multiple windows and open up the same file in different bands this is useful to identify different objects. Q1: Briefly…

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Nt1310 Unit 3 Lab Report

Basics of a-Si:H/uc-Si:H tandem solar cells Figure 1.2 shows the scheme of the one of the possible configuration for the thin-film tandem solar cell, which consists out of three blocks: the upper thin-film part, the thick layer of Silicon wafer and the bottom thin-film part, which are built in stack. Figure 1.2. The schematic view of the HIT solar cell According to Schockley the energy of photons, which exceeds the band gap is lost due to the thermalization of the charge carriers to the band edges…

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Nt1310 Unit 3 Lab Report

flow rate. The fluid flows through hose pipes (0.5in.) by a pump (0.5 hp and 30-40 m head) from the tank to the radiator at the flow rate range 500-550 LPM. The total volume of the circulating fluid is 3lts and constant in all the experimental steps. 3.…

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Nt1310 Unit 3 Lab Report

Diaphragm h. Coarse focusing knob i. Illuminator j. Base ~~5. What type of microscope has primarily been used in this lab? (1 point) The compound light microscope was used in this lab. ~~6. State the function of the following parts of the microscope: (3 points) A. mechanical stage- This is the area where a specimen is placed on the microscope for viewing. B. ocular lens- This is used to magnify the image that has been formed…

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Nt1310 Unit 3 Lab Report

3.1.5.3. Coverslip culture technique Coverslip culture technique was used for reviewing the morphological characteristics of the marine actinomycetes isolates (Pridham et al., 1958). Sterile glass cover slips were inserted at an angle of 450 into solidified SCA medium in petri plate. A loopful of inoculum of each isolate was streaked laterally to the line where the coverslip come across the agar and then the plates were inoculated at room temperature (28±200C) for 7 days. After the development of…

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Nt1310 Unit 3 Lab Report

10. When will you be required to use the spill kit in a healthcare setting?  The spill kit is used for cleaning the spills like bloods, body fluids, chemical spills or other unknown liquid spills. 11. You walk into the patient’s room and find that the person’s Catheter bag is leaking and there is urine on the floor beside the patient’s bed. Describe how you would respond and what is the process for cleaning up the spill?  The procedure for cleaning up a leaking catheter bag is: • Must wear appropriate…

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Nt1310 Unit 3 Measurements Lab Report

Purpose: The purpose of this lab is to be able to properly use all units of the metric system, and be able to convert the metric units into those that are similar. In order to identify which metric system is being used, it is determined by the measuring device in which is supplied: Thermometers read degrees celsius, graduated cylinders read volume; milliliters, and two forms of weight devices similar to scales called Analytical Balance and Triple Beam Balance in which measure mass. When reading,…

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