Iodine Test

Submitted By jake_up94
Words: 590
Pages: 3

TEST FOR STARCH
Iodine test
Method:
First, we took a test tube and added 2cm³ of 1% starch solution. Then we added a few drops of iodine solution into the same test tube. Both the solutions were initially colourless, but the minute they both come in contact they reacted to give out a blue-black colour.

Material List:

1. Starch solution

2. Iodine solution
3. Test tube

4. Test tube rack

5. Goggles

6. Gloves

7. test tube

8. Pipette

9. Test tube holder

TEST FOR LIPID
Emulsion Test:
Method:
First, we took a screw top test tube and added 2cm³ of oil. Then we added 2cm³ of absolute ethanol into the same test tube. Then we closed the top of the screw top test tube. Then we shook the test tube vigorously. After this, we unscrewed the test tube and added an equal amount of water. The oil we added was yellow in colour and the ethanol was colourless. However, when we mixed them, they form some sort of tiny bubbles; soon they get cloudy and form a milky white colour. Then when we added the cold water, then we can see two layers forming inside the test tube, where the oil floats on top and water sinks below because the density of water is much higher than oil.
Materials Required:
1. Oil

2. Absolute Ethanol
3. Test tube

4. Test tube rack

5. Goggles

6. Distilled water
7. test tube

8. Pipette

9. Test tube holder

10. Screwed top test tube

TEST FOR PROTEIN
Biuret Test
Method:
First, we added 2cm³ of protein solution to a test tube. Then we added an equal volume of 5% potassium hydroxide solution and mix. Then we mix this solution and then add two drops of 1% copper sulphate solution and mix. When we mixed these solutions together, the colour changed to purple.

Material List:
1. Protein solution

2. Potassium Hydroxide
3. Test tube

4. Test tube rack

5. Goggles

6. Gloves
7. Test tube

8. Pipette

9. Test tube holder

10. Copper sulphate

TEST FOR REDUCING SUGAR
BENEDICTS TEST
Method:
First, we added 2 cm³ of a solution of the reducing sugar to a test tube. Then we added an equal volume of Benedict’s solution, the solutions colour was blue. We named this test tube (A). Next, we had to repeat the same experiment again but this time we did the test with another solution of the reducing sugar and named it (B). The next step was to keep both the test tubes that contained the