Amylase Lab Report

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Introduction In chemical reactions, enzymes, which are mainly proteins, serve as catalysts, meaning they expedite reactions without being depleted. Enzymes catalyze reaction by lowering the activation energy barrier. Each enzyme has a specific shape and contains an active site. A substrate binds to this active site and is held by a weak hydrogen or ionic bond. In this lab Amylase, a type of digestive enzyme, was used to break down starch from a polysaccharide, into maltose, a disaccharide. The purpose of the lab was to determine how environmental factors affect the efficiency of enzymes. Enzyme efficiency can vary depending on different environmental factors such as, temperature, pH, and salt concentration. Enzymes are affected by temperature because at certain temperatures enzymes move more, which increases the rate at which they bind with their substrate. The optimal temperature for enzymes is 35-40 degrees Celsius. This is true with pH also, expect the optimal pH values for enzyme function is 6-8 pH. With salt concentration, the enzyme can denature and become inactive. …show more content…
The following question was asked: How does salt concentration affect how efficiently amylase breaks down starch? We hypothesized that Amylase will break down starch less effectively when the salt concentration is higher. We made this hypothesis based on our knowledge that blood cells have a salt concentration of 0.9 percent. The optimal salt concentration would be under 0.9 percent, since blood cells function at 0.9 percent. We hypothesized that the cells would not be able to function at a higher concentration, which lead us to our hypothesis. We predicted that if our hypothesis was correct the absorbance would increase as the salt concentration

Amylase And Starch Lab Report

to the “Enzymes-The Catalysts of Life” Lab (Flinn Scientific), pages 2-3 for the proper procedure. Data- see attached sheet Graph- see attached sheet Results and Discussion- Effects of Amylase on Starch: In this experiment, 4 test tubes were tested with benedict’s solution. This solution will turn the substance orange if sugars are present. One tube was filled with starch, another filled with glucose, one containing starch and the enzyme, amylase, and finally a tube filled with distilled…

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B-Amylase Lab Report

Slight Decrease of the enzyme will favor the B-amylase, while allowing the a-amylase, amylopectin breakdown, it will increase the fermentability, for the reason of maltose breaking down. Hypothesis: Slight Decrease of temperature during the mashing in the brewing process will affect the enzyme activity and the alcohol production compared to the control experiment. The enzymes will favor the b-amylase, as it allows some of a-amylase to breakdown. This will increase the fermentability. The Fermentation…

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Johan Hsu Lab Section 13154 Title: The Temperature Effect on the Interactions Between Porcine Pancreas Alpha-Amylase and 1% Starch Solution Introduction: Enzymes are virtually the additional power source that allows the continuation of life. The incredible powers of enzymes are conducted by complex protein molecules that are biological catalysts (Ophardt, 2003). Enzymes can vary vastly in the biological world by being categorized for performing different functions. Specifically, the porcine pancreas…

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Result Outline Essay

Angela Wang BIO 110 Enzymes II Lab Outline Effects of pH on the Breakdown of Starch by the Enzyme Amylase Result: Comparing the starch concentrations in different pH solution after 1, 3, 5, 7, and 9 minutes, the best pH environment for amylase to work efficiently appeared to be around pH 6 and pH 7 (see Figure 1.) ● Figure that shows the relationship between pH environment and the performance of amylase activity. ● Description of the trends, how different pHs resulted differently…

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How Does An Increase In Temperature Affect Kinetic Energy And Enzyme Activity

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Basic Chemistry For Investigating Living Systems

Shake well and allow the amylase to dissolve before proceeding. Discard any distilled water remaining in the beaker. Store the alpha-amylase dropper bottle solution in a refrigerator when not in use to reduce the rate of bacterial degradation. (Vorndam, 2002, p. 97-98) Calculation example for preparing a 1% solution: 0.10 g alpha-amylase powder is ~ equivalent to 0.10 mL when mixed in water, so 0.10 mL ÷ 10 mL x 100 = 1%. (Vorndam…

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