Ap Bio Lab

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Hannah Worden Lab 1 Writing Report BIOL:1411:0A26 03/07/24 Team Members: Olivia Blake, Kiyah Daily, Emily Schmit Experiment 1: Question and Hypothesis Big question: The big question for this experiment was, “After the centrifugation, which cell homogenate fraction would contain the highest amount of mitochondrial enzyme with the biochemical marker of succinate dehydrogenase?” Hypothesis: It was hypothesized that after all rounds of centrifugation, P3 would have the highest amount of the mitochondrial enzyme. Reasoning for Hypothesis: The reasoning for why P3 would have the highest amount of mitochondrial enzyme after the centrifugation took place was because it showed to be the largest in size, meaning it was more dense. In this experiment, …show more content…
We were able to test our hypothesis and find that it does make an impact when the pH level is higher. In graph one it is shown that pH 8 and pH 8.5 have the lowest amounts because the absorbance values are below 0. At pH 8.5 there was no change in absorbance over the 10 minute time interval and it continued to stay at -0.3. Overall, this graph helped show that the pH level does make a difference in the change in absorbance at 600nm over time. The different pH values were used as the independent variables for this experiment, while the pH 7 cuvette was used as the control variable. Each one of the cuvettes contained DCIP, sodium azide, assay buffer, succinate, and the P3 cell fraction so that we could properly find the date for the experiment. We used the P3 cell fraction in each of the cuvettes because that was what we had determined in Experiment 1 to contain the most of the mitochondrial enzyme.When using the spectrometer, we were able to measure the absorbance values and the change that would occur over the 5 minute intervals. After the experiment was over, we were able to look at the cuvettes and see if they had any color changes when coming out of the spectrometer. The cuvettes containing pH 8 and pH 8.5 have a blue color. The other four cuvettes had a color change to a clear and yellowish tint. This helped us conclude that what we had predicted was correct and that the 8 and 8.5 were going to stay blue and not turn colorless. Overall we achieved the goal of the experiment 2 and were able to collect proper data to support our big question and
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