Recognition Of The Promoter By DNA Polymeulatory In Nature

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Long noncoding DNA, regulatory in nature
Long noncoding RNA’s,
2’ position there is hydroxyl group in RNA, it has a an effect in metabolism.

DNA Configuration
5’ 3’ Leftright or in the opposite direction but still 5’-3’

Promoter Region for bacterial genes
-10 and -35
-35: Recognition of the promoter by RNA Polymerase in where it makes it contact. It is a site-specific interaction. That is how RNA Polymerase recognizes the promoter.
Sigma Factor: RNA specific binding portion for the binding of RNA.

Concessive sequences: Ideal interactions for RNA polimeraze. As you go away the less actie the promoter will be. Most prefer (identify) nucleotide a certain position. TTGGCA
It is found by comparing promoters and seeing what do they have in common.

-10: Unwinding of the DNA strands. RNA polymerase has to separate them for unwinding to occur. It is called -10 because of its location from transcription start.

+1 is the transcription start.

The spacing in a promoter is very clinical.

Bacterial gene expression.

Promoter mutations that lead to decrease gene expression: Away from concessive sequences.

Being translated as it is being made: There are many RNA polymerases going at the same time. AUG: Translation initiation codon.

Prokaryotes have nuclear envelopes

Bacterial interactions are very simple but eukaryotes there is a big flexibility.

Eukaryotes:

TATA Box is 4 nucleotides

Biding of regulatory proteins, or DNA Binding complex that cause the nucleosomes to bind and be removing. There are activation factors that act with RNA polymerase. We only have the TATA to

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