Nt1310 Unit 1 Lab Report

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1.) Sticky ends are sites seen on single-stranded DNA or RNA molecules, having a nucleotide base sequence complementary to that of another strand. These are created when restriction endonucleases are used to unevenly cut, or cleave DNA at a specific sequence, allowing for the two strands to be connected by base pairing. These structures are produced in the laboratory with the use of restriction enzymes for cloning purposes. Scientists can choose from several places to cut the genetic sequence with varying restriction enzymes. Ligation enzymes are then used to insert new genomic sequences into the full plasmid itself. These mutated, or recombined, plasmids can then be placed into cells in order to develop and be used for a few purposes, including the addition of genes to mammalian genomes and cloning.
2.) a.) The best technique to use out of the list of possible choices would be the Northern Blot. …show more content…
Then I looked further at the characteristics of each technique and what exactly they look at. Northern blotting is performed by extracting RNAs and fractionating them based on size by gel electrophoresis. It is used very heavily in studies for gene expression such as in determinations of whether specific mRNAs are present in different cell or tissue types. This technique would allow for a side by side comparison between cells at different times or in different organisms which would be useful in this scenario to possibly determine characteristics of the alien
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Nt1310 Unit 1 Lab Report

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