Protein Synthesis Lab Report

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Pages: 3

The purpose of laboratory 10 is to examine DNA, A,to understand the two-step process of protein synthesis and to analyze genes who have recently shared ancestry by comparing their gene sequence, amino acid sequences, and chromosomes. The materials required are; a strand of DNA, protein fragment code chart, the four gene sequences, the five amino acid sequence, and the four images of the chromosomes attached to the handout.

In the first procedure, we will first use a single strand of DNA to the complementary sequence of DNA. We will then translate the two segments into mRNA strands. Lastly, we will have to find the mRNA strand that has a start and stop codon in its sequence and construct the protein fragment coded for by the sequence using the table provided.
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First, we will examine the for gene sequences provided and mark the differences among the sequences that we find. We will then discuss some questions/observations with our lab partner which are; what specific patterns do you notice if any? What could this pattern mean regarding the ancestry/relatedness of the four species? Lastly, we are going to make a hypothesis about the ancestry of the four species in the form of a phylogenetic tree, drawing this on a separate sheet of paper and explaining why we drew it this way.

The third procedure is about molecular phylogenetics using a coding sequence. First, we will examine the amino acid sequences provided by the manual to mark any differences among the sequences that we find. Lastly, we will use the differences in amino acid sequence to retrace the ancestry of these mammals. We will then make a hypothesis in the form of a phylogenetic tree and explaining it with