so as to relatively quantify our understanding of how inhibitors, in different concentrations, can affect the reactions between enzyme and substrate.
METHOD
This experiment utilised the methodology outlined in pages 22 – 24 of the UTS 91707 Pharmacology Practical manual (Rodgers, 2015), with the following amendments: recording the reaction times as seconds rather than minutes, thus affecting the calculated values for our relative velocities within our results; and sampling triplets of 250ul aliquots…
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